Babak Ghanbarzadeh; Sahra Bashiri; Hamed Hamishekar; Jalal Dehghan nia
Abstract
Introduction : The encapsulation of nutraceuticals in lipid based carriers, such as liposomes, can lead to increasing of bio-active ingredients bioavailability and controlled release, maintaining their stability in different environmental conditions and increasing solubility of hydrophobic active ingredients ...
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Introduction : The encapsulation of nutraceuticals in lipid based carriers, such as liposomes, can lead to increasing of bio-active ingredients bioavailability and controlled release, maintaining their stability in different environmental conditions and increasing solubility of hydrophobic active ingredients in aqueous conditions. Food grade liposomes are being increasingly used in food industry to delivery hydrophilic and hydrophobic components such as vitamin E and vitamin C, ascorbic asid, nutraceuticals, essential omega 3 fatty medium chain fatty acids-vitamine C, nisine, cartenoides, oleic acids, polyphenols include catechine, synamic acids.One of the hydrophobic nutrients with antioxidant and beneficial pro-vitamine property is beta-carotene, which its high hydrophobicity and sensitivity in different environmental conditions has limited using of it for foodstuff enrichment. In order to improve the characteristics of the lipid bilayer, cholesterol traditionally has been included in the lipid membrane. It is important in decreasing permeability and strengthening the membrane. People suffering from hyper-cholesterolaemia are encouraged to avoid foods containing cholesterol. Since the plant sterols are natural compounds found in plant cell membranes which help maintain the membrane integrity. Such as Gama oryzanol is combination of different of plant sterols that is used in the formulation of nanoliposomes in this study to improve the stability of bilayers. The principal aim of this study was to prepare beta-caroten encapsulated nano- liposome formulations as a mean to improve its aqueous dispensability and to study the effect of lecithin-phytosterol concentrations on the partical size, encapsulation efficiency (EE), zeta potential, turbibility of beta carotene loaded nano-liposomes to get the optimized formulation. Materials and methods: Preparing liposomes is being carried out using different methods one of which is a novel technique called is “Mozafari method” (based on heating method). This method is characterized by the absence of organic solvent for the solving of lipids.Non-toxicity of produced liposomes; rapid production and scalability are some of the advantages of Mozafari method over other methods of liposome production. In this study, the liposomal ingredients were added to a preheated (60 0C, 5 min) water, mixeture of beta-carotene, gamma oryzanol solution and glycerol (final concentration 3% v/v) were added. The mixtures volume increased by adding warm water until 50ml, the mixture was further heated 60 while stirring 1200 rpm for 50-60 min under nitrogen atmosphere.Results and discussion: Effect of different concentration of lecithin (20, 40, 75, 100, 150, 200 mg) on particle size and zeta potential of nano-liposomes with constant amount of beta carotene (4 mg) and gamma-oryzanol for different concentration of lecithin with ratio 1:14 w/w were evaluated. The Particle size of nano liposomes with different concentration of lecithin was obtained below 500nm andthe optimal concentration of lecithin was 100 mg that particle size was minimum (64-88 nm).The gamma-oryzanol is a natural phytostrol which is as stabilizer for liposome membrance and promoting agent of hardness of vesicles wall however, the particle size of liposomes were reduced especially in low concentration of lecithin. The using phytosterols (gamma oryzanol) for maintaining the stability of liposomal membranesystems caused to reducing of particle size from 88nm to 64nm in 200 mg concentration of lecithin.The entrapment efficiency increased by increasing concentration of lecithin for nano-liposomes. It is because increasing the lecithin concentration, more vesicles were produced which in turn increased the loading capacity of nano-liposomes. In the liposome structure, the aqueous core and bilayer are the hydrophilic and hydrophobic parts, respectively. Therefore, the phospholipid bilayers place for beta carotene, and other hydrophobic substances. The entrapment efficiency in different concentration of lecithin was between 27-98%. The entrapment efficiency of liposomes containing beta carotene that used gamma oryzanol was less than liposomes without gamma oryzanol probably because the position of capsulation of gamma oryzanol and beta carotene is same in the bilayer of liposome that’s hydrophobic source of liposomes. But Gamma oryzanol was not effective on encapsulation efficiency of beta-carotene.The zeta potential, the electric potential in the interface or particle surface charge, is used to predict the stability of colloidal systems. In general, higher zeta potential values, regardless of their positive or negativity, indicate a higher and longer-term stability of the particles. Zeta potential of liposomes, which is a measure for the electrostatic repulsion and stability, was -29 and -35 milivolt for samples with and with not containing gamma oryzanol, respectively.For turbidity of liposomes, encapsulation of bioactive compounds can change the optical appearance due to the fact that the refractive index at the interface between solvent and internal phase changes and the size of liposomes may be altered. Increasing significantly of turbidity of liposomes (16% -80%), the wave length increase from 0.116 to 0.585 cm-1 high concentration of lecithin maybe due to increasing visuals and hydrophobic interactions.
Ladan Rahimi; Babak Ghanbarzadeh; Jalal Dehghan nia
Abstract
Introduction:Polyethylene terphthalate (PET) is one of the materials that are widely used for packaging of beverages and edible oils. In this study, the migration of di(2- ethylhexyl) phthalate (DEHP), dimethyl phthalate (DMP), diethyl phthalate (DEP), di-iso-butylphthalate (DIBP) and di-n-butylphthalate ...
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Introduction:Polyethylene terphthalate (PET) is one of the materials that are widely used for packaging of beverages and edible oils. In this study, the migration of di(2- ethylhexyl) phthalate (DEHP), dimethyl phthalate (DMP), diethyl phthalate (DEP), di-iso-butylphthalate (DIBP) and di-n-butylphthalate (DBP) from PET bottles into the lemon juice was investigated. Material and methods:According to European Commission regulations, 3% acetic acid (w/v) was chosen as simulant. The acetic acid samples were stored at 5, 25, and 40°C for three months and analyzed periodically by gas chromatography. Results and discussion: It was concluded that the storage temperature and time had a large effect on the migration of phthalet ester. The concentrations of migrating substance were more than its specific migration limit. The release kinetics of phthalet ester from PET bottle was described using Fick’s second law of diffusion coefficient, and crank model. The diffusion coefficients (D) determined for DEHP have most migration than other, were 0.084, 0.109, and 0.159 ×10-9 cm2/s at 5, 25, and 40°C, respectively. The diffusion coefficients (D) determined for DBP have most migration than other, were 0.084, 0.105, and 0.138 ×10-9 cm2/s at 5, 25, and 40°C, respectively. The diffusion coefficients (D) determined for DIBP have most migration than other, were 0.084, 0.177, and 0.125 ×10-9 cm2/s at 25, and 40°C, respectively. The temperature dependence of D, Arrhenius equation giving values of activation energy (Ea) for DIBP, DBP and DEHP are 2.82, 2.06 and 1.302 J mol-1 respectively.
Jalal Dehghan nia; Hamed Bagheri-Darvish-Mohammad; Babak Ghanbarzadeh
Abstract
Introduction: Deep-fat frying is a process of cooking foods through immersing them in edible oils at temperatures above the boiling point of water (150-200°C). During this complex unit operation, heat and mass transfer occur simultaneously.During frying, heat is transferred from edible oil to surface ...
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Introduction: Deep-fat frying is a process of cooking foods through immersing them in edible oils at temperatures above the boiling point of water (150-200°C). During this complex unit operation, heat and mass transfer occur simultaneously.During frying, heat is transferred from edible oil to surface of the food and then transferred into it and at the same time, moisture is transferred from inside the food to outside.As a result of these phenomena and by continuing the process, food temperature increases and its moisture content decreases. This, in turn, creates favorable characteristics such as color, texture and taste of the product.Moisture content is one of the important features in the quality of fried products.In the frying process, moisture loss from food occurs by the mechanisms of molecular diffusion, capillary flow and pressure driven flow.The driving force of moisture loss is the partial water vapor pressure difference between the inside and the surface of the food product due to turning the water into vapor.Rate of moisture loss from the food during the frying process decreases exponentially with frying time and increases with increasing temperature.For information about therelationshipsbetweenvarious variables during the frying process, moisture loss kinetics modelingcan bea suitable steptowards improving thequality offried products.To our knowledge, there has been no study in literature associated with the effect of ultrasound and microwave on moisture loss during deep-fat frying of foods. This study aimed to evaluate the effect of these waves on moisture loss kinetics during frying of potato strips.Materials and Methods: Potatoes (Agria variety) were purchased from a local market and kept in a cold room at 0°C. A mixture of sunflower, soy and cottonseed oil (Behshahr Industrial company), was used for frying potato strips.Inthis study, effect ofultrasound pretreatment at frequencies of 28 and 40 kHz for 15 min and microwave pretreatment at powers of 3 and 6 W/g for 10 min on moisture content of the fried potato slices at 150, 170 and 190°C for 60, 120, 180 and 240 s was investigated.The moisture content of the samples was measured by drying them in a convection oven at 105°C until the weight was constant.Moisture loss experimental data during frying were fitted with six empirical models proposed in this study as well as the Fick’s law of diffusion.The effective moisture diffusion coefficient was calculated based on the Fick's law. To calculate the effect of temperature on the effective moisture diffusion coefficient, the Arrhenius equation was used.Results and Discussion: By increasing frying temperature, moisture content of the potato slices decreased; however the decrease was not significant at a probability level of 5 percent. The positive effect of oil temperature on moisture loss during deep-fat frying of potato strips has been well documented. This is due to the high kinetic energy of water molecules at higher temperatures, leading to a rapid loss of moisture. The moisture loss by diffusion of water molecules as well as the oil uptake during the frying process lead to the formation of cracks in the structure of the solid food. This, in turn, leads to structural damages and significant changes in terms of structural characteristics including porosity.On the other hand, moisture content of the samples significantly decreased in an exponential manner by increasing the process time. Rapid moisture loss in the first moments of frying is associated with the removal of surface moisture. By decreasing surface moisture over time, the rate of moisture loss was reduced accordingly.Results also showed that both the ultrasound and microwave pretreatments at all the studied levels significantly reduced the final moisture content of the samples at a probability level of 5 percent. The difference between the samples pretreated with two ultrasound frequencies of 28 and 40 kHz was not significant (P > 0.05), but with increasing frequency of the pretreatment, the moisture content decreased to a greater extent. Lower final moisture contents of the samples pretreated with ultrasound were probably due to the creation of microscopic channels in the food structure, which may facilitate moisture loss during frying. On the other hand, application of microwave pretreatment at powers of 3 and 6 watts per gram, decreased initial moisture content of the samples by 38 and 80%, respectively. This resulted in significant (P < 0.05) reduction of the final moisture contents of the samples pretreated with microwave. More moisture loss at higher microwave power is probably due to the high intensity of electromagnetic energy as a result of microwave volumetric heating.In addition, the applied modelswerewell fitted toexperimentaldata having high R2 and low RMSE. The effective moisture diffusion coefficient ranged between 3.57×10-8 to 11.08×10-8 m2/s. Results also demonstrated that the effective moisture diffusion coefficient is increased and the activation energy is decreased by implementing the ultrasound and microwave pretreatments.
Ronak Gholami; Jalal Dehghan nia; Babak Ghanbarzadeh
Abstract
Introduction: In recent years, demand for edible and biodegradable films has increased. One reason for this increase is the pollution caused by synthetic polymers. Edible films are produced from different biopolymers such as lipids, polysaccharides and proteins. Starch is a common polysaccharide in the ...
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Introduction: In recent years, demand for edible and biodegradable films has increased. One reason for this increase is the pollution caused by synthetic polymers. Edible films are produced from different biopolymers such as lipids, polysaccharides and proteins. Starch is a common polysaccharide in the preparation of edible films which is taken into consideration because of its low price and easy access. Structure and composition of starch-based films affects the resulting film properties such as moisture sorption, gas permeability, plasticizer crystallization, glass transition temperature and its mechanical properties. Starch films have usually poor mechanical properties and are permeable to water vapor. The use of nanofillers such as cellulose nanocrystal (CNC) in the structure of starch films and production of nanocomposite films is one way to modify properties of the films. The most important purpose of the application of edible films is to prevent moisture or other compounds such as carbon dioxide or volatile components transfer between the product and the environment or between different layers of the product. Modeling mass transfer and moisture permeability of edible films can be effective in predicting film properties and packaged product during storage. For example, it can be predicted that at a certain temperature, relative humidity and time, how much moisture packaging material will absorb. Therefore, before using edible film as a protective coating for food, calculation of the amount of moisture sorption and permeability to water vapor is essential. The purpose of this study was to investigate mass transfer in starch - CNC nanocomposite films. The effect of adding different percentages of CNC on the water vapor permeability and moisture sorption kinetics of nanocomposite films was studiedMaterials and Methods: First, 100 ml of potato starch solution with a concentration of 4% (w/v) was prepared by dispersion of the starch in distilled water and was gelatinized at 90ºC for 5 min. Different levels of CNC (0, 3, 5, 7 and 9% w/w) were dissolved in distilled water and were added to the gelatinized starch after treatment with ultrasound for 10 min. Then, glycerol, as a plasticizer, with concentrations of 0.2, 0.3 and 0.4% (w/w) were added to the solution. The film solutions were distributed on polystyrene surfaces and the resulting films were dried in an oven at 40°C for 24 hours. The Fickʹs second law and four empirical equations were used for moisture sorption modeling of samples. The effect of glycerol concentration on water vapor permeability was investigated and the experimental data were fitted with an exponential model.Results and Discussion: By increasing the concentration of CNC, moisture content of the nanocomposite films declined. Effective moisture diffusion coefficient values for nanocomposite samples were higher than the pure starch film. The coefficient increased from 0.293×10-13 to 0.547×10-13 m2/s by increasing CNC concentration from 0 to 9%. This result can be attributed to the influence of cellulose nanofibers on the polymer matrix and gaps creation in the polymer amorphous regions. This, in turn, would facilitate moisture diffusivity into the polymer structure. It should be noted that plasticizer presence in the nanocomposite structure can be an important factor. Regarding that plasticizer lead to increase in polymer chain mobility, simultaneous presence of CNC and plasticizer could lead to create gaps in the structure of nonocompositefim. As expected, in the absence of plasticizer, the effective moisture diffusion coefficient in nanocomposite samples decreased by increasing the concentration of nanoparticles due to high immobility of polymer chains. In addition, the initial stages of moisture sorption were well described by the Fickʹs law but due to the polymer relaxation between 2.5 - 9 h interval, its behavior was deviated from this law. Finally, after about 9 hours, it was observed that the equilibrium moisture content of the nanocomposite samples were consistent with the values predicted by the Fickʹs model. Equilibrium moisture content depends on the hydrophilic locations of the nanocomposite structure. These locations have the ability to absorb moisture and this ability is not influenced by changes in the structure of the polymer during the moisture sorption process. Despite higher levels of effective moisture diffusion coefficients in starch-nanocrystalline cellulose nanocomposites compared to pure starch film, moisture content was lower in nanocomposite films. These results are probably due to the nature of nanocrystalline cellulose which is resistant to water and is compatible with the starch polymer. Nanocrystalline cellulose has the ability to make many hydrogen bonds with the hydrophilic polymer matrix. This results in decreasing hydrophilic property of starch. On the other hand, in all samples, the permeability to water vapor reduced with increasing nanoparticles concentration. For example, in the starch film which contained 0.4% glycerol, water vapor permeability was 2.62×10-7g.m/m2.h.Pa; with the addition of nanocrystalline cellulose to 9%, its value was decreased to 1.8×10-7g.m/m2.h.Pa. Moreover, the permeability to water vapor in all cases increased by increasing the concentration of plasticizer. Results also showed that there is an exponential relationship between the water vapor permeability and plasticizer content.Conclusion: By increasing the concentration of CNC, moisture content of the nanocomposite films declined. Effective moisture diffusion coefficient values for nanocomposite samples were higher than the pure starch film. The coefficient increased by increasing CNC concentration. The initial stages of moisture sorption were well described by the Fickʹs law but due to the polymer relaxation, its behavior was deviated from this law. Finally, after about 9 hours, it was observed that the equilibrium moisture content of the nanocomposite samples were consistent with the values predicted by the Fickʹs model. In addition, in all samples, the permeability to water vapor reduced with increasing nanoparticles concentration. However, the permeability to water vapor increased by increasing the concentration of plasticizer. Results also showed that there is an exponential relationship between the water vapor permeability and plasticizer content
Hadi Almasi; Babak Ghanbarzadeh; Jalal Dehghan nia; Ali Akbar Entezami; Asghar Khosrowshahi Asl
Abstract
Fatty acid modified cellulose nanofibers (MCNFs) and TBHQ antioxidant were added to poly(lactic acid) (PLA) film. The combined effects of the MCNFs and TBHQ on the morphological, thermal, mechanical and barrier properties of PLA film were analyzed. The morphology of fracture surfaces evaluated by field ...
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Fatty acid modified cellulose nanofibers (MCNFs) and TBHQ antioxidant were added to poly(lactic acid) (PLA) film. The combined effects of the MCNFs and TBHQ on the morphological, thermal, mechanical and barrier properties of PLA film were analyzed. The morphology of fracture surfaces evaluated by field emission scanning electron microscopy (FE-SEM). XRD results showed that the crystallinity of the PLA film with added MCNFs was substantially higher than that of pure PLA and antioxidant active PLA films. Glass transition and melting temperatures changed with the addition of these two components. The addition of 3wt% of TBHQ to PLA films leads to a significant reduction (p
Mahdi Barmour; Jalal Dehghan nia; Babak Ghanbarzadeh
Abstract
The objective of this study was to evaluate the effect of process conditions and different pretreatments including ultrasound, microwave and osmotic dehydration on mass transfer and oil uptake during deep fat frying of potato slices. Ultrasound pretreatment was performed at frequency of 40 KHz for 10 ...
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The objective of this study was to evaluate the effect of process conditions and different pretreatments including ultrasound, microwave and osmotic dehydration on mass transfer and oil uptake during deep fat frying of potato slices. Ultrasound pretreatment was performed at frequency of 40 KHz for 10 and 30 minutes, microwave pretreatment was conducted at 5 W/g power and osmotic dehydration pretreatment was done in NaCl solutions with concentrations of 1 and 3 percent. Potato slices were then fried at 150, 170 and 190°C for 90, 180, 270 and 360 seconds. The results showed that ultrasound pretreatment for 10 minutes increases oil uptake of samples as compared with control sample, but when samples were exposed to ultrasound for 30 minutes, oil uptake was decreased. In addition, microwave pretreatment reduced oil uptake of potato slices insignificantly. Furthermore, osmotic dehydration pretreatment reduced oil uptake. In order to model oil uptake, experimental data were fitted with 6 models. The aforementioned models had the highest R2 and a minimum value of RMSE.
Nasrin Jamshidi; Babak Ghanbarzadeh; Jalal Dehghan nia; Mahood Sowti Khiabani; Ali Akbar Entezami
Abstract
Cellulose nanocrystal (CNC) is a type of nanomaterial which is produced by partial hydrolysis of cellulose and elimination of its amorphous regions. CNC has several advantages such biodegradability and safety toward human health. In this study, CNC was produced from cotton linters and methods such transmission ...
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Cellulose nanocrystal (CNC) is a type of nanomaterial which is produced by partial hydrolysis of cellulose and elimination of its amorphous regions. CNC has several advantages such biodegradability and safety toward human health. In this study, CNC was produced from cotton linters and methods such transmission electron microscopy which were used for confirmation of nanoscale size production of cellulose crystals. Due to the disadvantages of pure starch films, in the present research, for improving the properties of plasticized starch-PVOH films, from nanoparticles CNC and TiO2 are used together, then effects of nanoparticles and glycerol determined on physical properties by response surface methodology (RSM). CNC and GLY showed significant linear effects on ultimate tensile strength (UTS) of nanocomposit and there were significant interaction effects between TiO2 and CNC, and also between GLY and TiO2. The optimum levels of TiO2, CNC and GLY for obtaining maximum UTS were as 0.118, 0.6 g and 1.06 ml, respectively. In addition, the TiO2 concentration had linear and quadratic effect on the contact angles of bionanocomposites and optimum levels of TiO2, CNC and GLY for obtaining maximum contact angles were 0.112, 0.299 g and 1.06 ml, respectively. UV-visible spectroscopy studies in the wavelength range 200–800 nm showed that adding of CNC and TiO2 decrease the light transmission and increase the opacity, adding of glycerol increase the light transmission and decrease the opacity.
Mina Akbarian; Babak Ghanbarzadeh; Jalal Dehghan nia; Mahood Sowti Khiabani
Abstract
In this study, the optimization of osmotic solutions (containing fructose, calcium chloride and citric acid) were investigated based on maximum water loss (WL) in the osmotic dehydration of quince. The response surface methodology (RSM) and central composite design, with 18 treatment and 3 replicate, ...
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In this study, the optimization of osmotic solutions (containing fructose, calcium chloride and citric acid) were investigated based on maximum water loss (WL) in the osmotic dehydration of quince. The response surface methodology (RSM) and central composite design, with 18 treatment and 3 replicate, was used for optimizing. The results showed that fructose and calcium chloride had linear and quadratic significant effects (p
Leila Abolghasemi Fakhri; Babak Ghanbarzadeh; Jalal Dehghan nia; Ali Akbar Entezami
Abstract
The high hydrophilic property of biopolymer based plastics is one of the most important defects of them. Blending biopolymers with compatible synthetic polymers and using nano particles such as nanoclay as nanofiller are improving methods that have been extensively considered in recent years. Carboxymethyl ...
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The high hydrophilic property of biopolymer based plastics is one of the most important defects of them. Blending biopolymers with compatible synthetic polymers and using nano particles such as nanoclay as nanofiller are improving methods that have been extensively considered in recent years. Carboxymethyl cellulose (CMC) is one of the lowest cost biopolymers that have enormous applications in different industries and polyvinyl alcohol (PVA) is a synthetic polymer which has a high compatibility with biopolymers. In this research, CMC-PVA nanocomposites containing 3-10% Montmorillonite (W/W CMC) were prepared by casting method. The X-ray diffraction (XRD) results indicated the formation of an exfoliated nanostructure in all MMT content samples. Nanocomposites containing 10% MMT exhibited 29.06% reduction in water vapor permeability (WVP) compared to the blend film without nanoclay. Samples with 10% MMT, showed the lowest surface hydrophilisity (contact angle = 65.1o). The differential scanning calorimetry (DSC) results indicate that by increasing of MMT contents, the glass transition disappeared gradually and no glass transition was observed in the thermograms of the films containing 5, 7 and 10% MMT.
